In vitro

  • Cell cultures of rodent neural stem cells neurons, astrocytes, oligodendrocytes, microglia, macrophages, mesenchymal stem cells exposed to different insults (IL-1beta, IL-6, TNF-alpha, LPS, Poly:IC, beta-amyloid, different glutamate analogues).
  • Co-cultures of two types of rodent primary cells, with or without mechanical contacts, exposed to different insults.
  • Primary cell cultures of human neural stem cells, microglia, astrocytes, and human mesenchymal stem cells exposed to different insults.
  • Human induced pluripotent cells (IPS) cells from controls and patients with genetic neurodevelopmental disorders : 2D and 3D – brain organoids – cultures.
cell phenotyping, survival, proliferation, and differentiation; production of cytokines-chemokines, ROS, extracellular vesicles, trophic factors, energy metabolism (Seahorse)


  • Microglia-specific CRE mouse lines
  • Encephalopathy of prematurity: systemic administration of IL-1beta or LPS, intracerebral administration of glutamate analogues.
  • Traumatic brain injury: close head impact during brain development.
  • Double-hit (inflammation + delayed traumatism, excitotoxicity, or inflammation) models
  • 3-DNA nanoparticles to specifically target microglia (mouse and rat)
neuropathological evaluation; IF and quantification; RNAscope; brain and immune cell phenotyping (FCAS); bulk RNA-seq on sorted (MACS) cell populations; scRNA-seq: spatial transcriptomics (Visium technology); blood-brain barrier integrity; behavioral tests (motor and cognitive performances, social interactions, anxiety, aggressivity) during development and adulthood; functional brain connectivity (functional ultrasound)


  • Pediatric CIC, Biological resource center (including post-mortem brain tissues), stratified cohorts of patients
MRI imaging, high density EEG, neurological, neuropsychological and behavioral phenotyping, immune phenotyping (FACS), genotyping.
For a complete panorama of our offer regarding neurodevelopmental disorders, click here.
  • Fundamental research


  • Pre-clinical research
  • Clinical research